WesternBright Sirius HRP substrate

 

WesternBright Sirius HRP substrate

WesternBright Sirius is the most sensitive HRP substrate available from Advansta for chemiluminescent Western blotting. With attomole sensitivity and a long-lasting signal, WesternBright Sirius allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.

WesternBright Sirius is the most sensitive of the HRP substrates

WesternBright Sirius is designed for sensitive detection and low antibody concentrations. With low background and a strong, long-lasting chemiluminscent signal, WesternBright Sirius is the best choice for quantifying low-abundance bands.

WesternBright Sirius provides a strong chemiluminscent signal

WesternBright Sirius is the most sensitive chemiluminescent HRP substrate offered by Advansta, specially engineered to produce an extremely strong signal to detect low-abundance proteins and to work with very low antibody concentrations.

High sensitivity, low background

 

 

Sensitivity of chemiluminescent substrates from various suppliers. 2-fold serial dilutions of purified transferrin were electrophoresed by SDS-PAGE then transferred to a PVDF membrane. The blots were blocked then probed with a rabbit anti-transferrin primary antibody followed by an HRP- conjugated anti-rabbit secondary antibody. Various substrates were applied to the membranes then signal was measured with a CCD camera.

 

Image blots hours after substrate incubation

A slot blot containing a serial dilution of an HRP-conjugated antibody was incubated with WesternBright Sirius, and imaged at the times indicated. After 8 hours, the bands are still easily detected with a 2 minute exposure.

Low background for sensitive detection and better quantitation

WesternBright Sirius provides sensitive detection combined with extremely low background. Duplicate Western blots were performed with samples of plasma, diluted either 1:100 (left lanes) or 1:600 (right lanes) in PBS, and probed with a goat antisera primary antibody and an HRP-conjugated secondary antibody. After detection with either WesternBright Sirius or SuperSignal® West Femto (Thermo Scientific), the blots were imaged for 8.3 minutes. The signal-to-noise ratio is shown for each band.

 

Customer feedback

"WesternBright Sirius performed better than Pierce's Femto and had much less background, while maintaining similar (if not better) sensitivity. It is also working better for my specific home-made antibodies."

- Postdoctoral Researcher, Indiana University

"The WesternBright Sirius substrate has low background and we are able to visualize phosphorylated proteins that we had difficulty seeing with our previous substrates. We have been able to dilute our primary antibodies from 1:1000 to 1:2500 - 1:5000."

- Graduate Research Associate, Ohio State University

"The signal lasts much longer with WesternBright Sirius, while the Pierce West Femto signal disappeared after a half an hour. Plus, WesternBright Sirius is less expensive!"