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美国polysciences公司现货供应

Polysciences 是一家化学品制造公司,产品广泛应用于各个科研领域。公司成立于1961年,起初为电子显微镜提供纳米级样本制备方案,帮助科学家揭示未知领域。随后,开拓了在病理(组织研究)应用产品,使组织除了石蜡包埋外,还能够包埋在塑料块中;开发染料和染色剂,以实现更好的样品观测方式。与政府机构合作,开发了用于诊断试剂盒应的聚合物微球。与美国国家癌症中心合作,开发天然产物分离和纯化产品,并用于紫杉醇的初步临床试验。继而开发出超顺磁珠,用于DNA、蛋白质和肽的研究。Polysciences的高纯度单体和聚合物产品在医疗器械中有许多应用,并不断开发和增强其性能。近期业务扩展到电子产品,Polysciences的精密微粒子产品拓展了纳米技术应用中测量精度。公司秉承为应用而研发,目前已拥有超过3000种产品。Polysciences Inc.致力于提供先进的技术、制造能力和技术支持,帮助客户实现他们的目标。
PEI 25K is a powerful, trusted, and cost-effective transient transfection reagent. In HEK293 and CHO expression systems, PEI offers consistently high gene expression on a wide scale (96 well plates up to 100 L bioreactors). Each year, more researchers and companies turn to Polysciences PEI to gain a critical edge in their work. Relative to most other options, using PEI to prepare transfection reagents in-house can offer as much as a 40% reduction in total transfection costs.
Polysciences
APPEARANCE:
White to yellow solid
MELTING POINT:
73-75º
MOLECULAR WEIGHT:
25,000
SOLUBILITY:
hot water, cold water at low pH, methanol, and ethanol.<br><strong>Insoluble In:</strong> benzene, ethyl ether and acetone

23966-1 Polyethylenimine, Linear (MW 25,000) 1G polysciences

24765-1 Polyethylenimine HCl MAX, Linear, Mw 40,000 (PEI MAX 40000) 1G Polysciences
转染试剂系列是基于聚乙烯亚胺(Polyethylenimine,PEI)的产品,因其较高的转染效果,已广泛应用于工业化生产。聚乙烯亚胺是一种具有较高的阳离子电荷密度的有机大分子,每相隔二个碳原子,即每“第三个原子都是质子化的氨基氮原子,使得聚合物网络在任何pH下都能充当有效的“质子海绵”体(proton sponge)。PEI,可用作转染试剂,它可以缩聚DNA分子形成颗粒并转染真核细胞。有实验证明,分子量为25000的线性PEI即Polysciences 23966(PEI 25K)转染效率表现优良。
产品特点:
1. 特别适用于生产 mABs、rAbs、bsABs和病毒载体(AVV、LV、BEV)
 
2.在HEK293、CHO 和 Sf9 细胞系中高度有效
 
3.适用于从研发到工业化生产的各个阶段
 
4.可预测,可大规模生产
 
5.两周内即可生成大量目标蛋白或病毒
 
6.高转染效率

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Bang, S., Li, J., Zhang, M., Cui, R., Wu, X., Xin, Z., ... & Zhang, H. (2020). The Clinical Relevance and Function of Krüppel-Like Factor 16 in Breast Cancer. Cancer Management and Research, 12, 6373.

Baranyi, L., Doering, C. B., Denning, G., Gautney, R. E., Harris, K. T., Spencer, H. T., ... & Dropulic, B. (2013). Rapid generation of stable cell lines expressing high levels of erythropoietin, factor VIII, and an antihuman CD20 antibody using lentiviral vectors. Human Gene Therapy Methods, 24(4), 214-227.

Durocher, Y., Perret, S., & Kamen, A. (2002). High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research, 30(2), e9-e9.

Li, J., Yu, W., Ge, J., Zhang, J., Wang, Y., Wang, P., & Shi, G. (2020). Targeting eIF3f Suppresses the Growth of Prostate Cancer Cells by Inhibiting Akt Signaling. OncoTargets and therapy, 13, 3739.

Sanzhez-Mata, A., Ferez-Gomez, A., & Gonzalez-Muñoz, E. (2020). Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs. STAR protocols, 1(3), 100183.

Schmitz, C., Pepelanova, I., Seliktar, D., Potekhina, E., Belousov, V. V., Scheper, T., & Lavrentieva, A. (2020). Live reporting for hypoxia: Hypoxia sensor–modified mesenchymal stem cells as in vitro reporters. Biotechnology and Bioengineering, 117(11), 3265-3276.

Siegman, S., Truong, N. F., & Segura, T. (2015). Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta biomaterialia, 28, 45-54.

Thomas, M., Lu, J. J., Ge, Q., Zhang, C., Chen, J., & Klibanov, A. M. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proceedings of the National Academy of Sciences, 102(16), 5679-5684.

Trefny, M. P., Kaiser, M., Stanczak, M. A., Herzig, P., Savic, S., Wiese, M., ... & Zippelius, A. (2020). PD-1+ natural killer cells in human non-small cell lung cancer can be activated by PD-1/PD-L1 blockade. Cancer Immunology, Immunotherapy: CII.

Wulhfard, S., Baldi, L., Hacker, D. L., & Wurm, F. (2010). Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of biotechnology, 148(2-3), 128-132.